Peptide Reconstitution Calculator

How the peptide reconstitution calculator works

This tool calculates the exact concentration of a reconstituted peptide and the syringe units that correspond to a given amount. Enter the peptide in the vial (mg), the volume of bacteriostatic water you are adding (ml) and the target amount, and it returns the resulting concentration in mg/ml plus the point to draw the syringe to.

What reconstitution means

Research peptides are supplied as a lyophilised (freeze-dried) powder. Reconstitution is the step of dissolving that powder in bacteriostatic water to reach a known, measurable concentration. The peptide amount never changes — only the volume of water determines how concentrated the final solution is.

How to use it

Select the total volume of your syringe (0.3 ml / 0.5 ml / 1.0 ml).
Enter the total peptide in the vial, in milligrams (e.g. 5 mg, 10 mg).
Enter the volume of bacteriostatic water you are adding, in millilitres.
Select the target amount — the tool shows the concentration (mg/ml) and where to draw the syringe.

The formula

Concentration (mg/ml) = Peptide (mg) ÷ Bacteriostatic water (ml)
More water with the same peptide amount gives a lower concentration; less water gives a higher one. To convert units: 1 mg = 1000 mcg.

Quick reference table

Peptide in vial	Bacteriostatic water	Concentration
5 mg	1 ml	5 mg/ml
5 mg	2 ml	2.5 mg/ml
10 mg	1 ml	10 mg/ml
10 mg	2 ml	5 mg/ml
10 mg	3 ml	3.33 mg/ml
15 mg	2 ml	7.5 mg/ml
15 mg	3 ml	5 mg/ml

Frequently asked questions

What is peptide reconstitution?
Dissolving a lyophilised peptide powder in bacteriostatic water to obtain a solution of known concentration.

Which water is used?
Bacteriostatic water (water with 0.9% benzyl alcohol) is the standard diluent used to reconstitute lyophilised peptides in research settings.

How do I calculate the concentration?
Divide the peptide amount (mg) by the water volume (ml). The result is the concentration in mg/ml.

Does adding more water make the solution weaker?
Yes. The peptide quantity stays the same, so a larger water volume produces a lower mg/ml concentration.

How do I convert mg to mcg?
Multiply by 1000: 1 mg = 1000 mcg.

CamoChem products and tools are intended exclusively for laboratory and research use. This calculator provides concentration mathematics only and is not medical, dosing or usage guidance. Not for human consumption.